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Protein digestion and phosphopeptide enrichment on a glass microchip

This work describes an integrated glass microdevice for proteomics, which directly couples proteolysis with affinity selection. Initial results with standard phosphopeptide fragments from β-casein in peptide mixtures showed selective capture of the phosphorylated fragments using immobilized metal af...

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Dades bibliogràfiques
Publicat a:Anal Chim Acta
Autors principals: Yue, Guihua Eileen, Roper, Michael G., Balchunas, Catherine, Pulsipher, Abigail, Coon, Joshua J., Shabanowitz, Jeffery, Hunt, Donald F., Landers, James P., Ferrance, Jerome P.
Format: Artigo
Idioma:Inglês
Publicat: 2005
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4631396/
https://ncbi.nlm.nih.gov/pubmed/17723369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.aca.2005.11.003
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