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AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities

High throughput sequencing (HTSeq) of small ribosomal subunit amplicons has the potential for a comprehensive characterization of microbial community compositions, down to rare species. However, the error-prone nature of the multi-step experimental process requires that the resulting raw sequences a...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Lange, Anja, Jost, Steffen, Heider, Dominik, Bock, Christina, Budeus, Bettina, Schilling, Elmar, Strittmatter, Axel, Boenigk, Jens, Hoffmann, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4629888/
https://ncbi.nlm.nih.gov/pubmed/26523925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0141590
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