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Optimal terminal sequences for continuous or serial isothermal amplification of dsRNA with norovirus RNA replicase

The norovirus RNA replicase (NV3D(pol), 56 kDa, single chain monomeric protein) can amplify double-stranded (ds) RNA isothermally. It will play an alternative role in the in vitro evolution against traditional Qβ RNA replicase, which cannot amplify dsRNA and consists of four subunits, three of which...

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Bibliografische gegevens
Gepubliceerd in:Biophysics (Nagoya-shi)
Hoofdauteurs: Arai, Hidenao, Nishigaki, Koichi, Nemoto, Naoto, Suzuki, Miho, Husimi, Yuzuru
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The Biophysical Society of Japan (BSJ) 2014
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4629653/
https://ncbi.nlm.nih.gov/pubmed/27493494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2142/biophysics.10.15
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