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Optimal terminal sequences for continuous or serial isothermal amplification of dsRNA with norovirus RNA replicase

The norovirus RNA replicase (NV3D(pol), 56 kDa, single chain monomeric protein) can amplify double-stranded (ds) RNA isothermally. It will play an alternative role in the in vitro evolution against traditional Qβ RNA replicase, which cannot amplify dsRNA and consists of four subunits, three of which...

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Detalhes bibliográficos
Publicado no:Biophysics (Nagoya-shi)
Main Authors: Arai, Hidenao, Nishigaki, Koichi, Nemoto, Naoto, Suzuki, Miho, Husimi, Yuzuru
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society of Japan (BSJ) 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4629653/
https://ncbi.nlm.nih.gov/pubmed/27493494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2142/biophysics.10.15
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