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Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
In this paper we describe the tools, reagents, and the practical steps that are needed for: 1) successful preparation of wholemount retinas for immunohistochemistry and, 2) calcium imaging for the study of voltage gated calcium channel (VGCC) mediated calcium signaling in retinal ganglion cells. The...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4621789/ https://ncbi.nlm.nih.gov/pubmed/25349920 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51396 |
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