Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases

Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases, and cellobiohydrolase. Diagnostic patterns for reactions of these different cla...

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Bibliographische Detailangaben
Veröffentlicht in:Front Bioeng Biotechnol
Hauptverfasser: Deng, Kai, Takasuka, Taichi E., Bianchetti, Christopher M., Bergeman, Lai F., Adams, Paul D., Northen, Trent R., Fox, Brian G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2015
Schlagworte:
Bioengineering and Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4621489/
https://ncbi.nlm.nih.gov/pubmed/26579511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2015.00165
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