Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis

Recent studies have shown that the Gram-positive bacterium Lactococcus lactis can be exploited for the expression of heterologous proteins; however, a versatile set of vectors suitable for inducible extracellular protein production and subsequent purification of the expressed proteins by immobilized...

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Detalhes bibliográficos
Publicado no:Appl Microbiol Biotechnol
Main Authors: Neef, Jolanda, Milder, Fin J., Koedijk, Danny G. A. M., Klaassens, Marindy, Heezius, Erik C., van Strijp, Jos A. G., Otto, Andreas, Becher, Dörte, van Dijl, Jan Maarten, Buist, Girbe
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2015
Assuntos:
Applied Genetics and Molecular Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4619460/
https://ncbi.nlm.nih.gov/pubmed/26160391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-015-6778-8
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