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ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo

Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the combination of ChEC with high-throughput sequencing (ChEC-seq) to map budding yeast transcription factor...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Zentner, Gabriel E., Kasinathan, Sivakanthan, Xin, Beibei, Rohs, Remo, Henikoff, Steven
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4618392/
https://ncbi.nlm.nih.gov/pubmed/26490019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9733
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