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ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo
Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the combination of ChEC with high-throughput sequencing (ChEC-seq) to map budding yeast transcription factor...
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Publicado no: | Nat Commun |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Nature Publishing Group
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4618392/ https://ncbi.nlm.nih.gov/pubmed/26490019 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9733 |
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