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In Situ Reverse Transcriptase-Nested Polymerase Chain Reaction to Identify Intracellular Nucleic Acids without the Necessity of Dnase Pretreatment and Hybridisation

In the present study a protocol of in situ reverse transcriptase‐nested polymerase chain reaction (in situ RT‐nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by “DNA repair mechanisms” and “endogenous priming”, a two‐step PCR was applied af...

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Bibliografiske detaljer
Udgivet i:Anal Cell Pathol
Main Authors: Menschikowski, Mario, Vogel, Margot, Eckey, Rolf, Dinnebier, Gerd, Jaross, Werner
Format: Artigo
Sprog:Inglês
Udgivet: IOS Press 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4618000/
https://ncbi.nlm.nih.gov/pubmed/11455034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2001/654016
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