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Detection and quantitative estimation of spurious double stranded DNA formation during reverse transcription in bacteria using tagRNA-seq

Standard RNA-seq has a well know tendency to generate “ghost” antisense reads due to formation of spurious second strand cDNA in the sequencing process. We recently reported on a novel variant of RNA-seq coined “tagRNA-seq” introduced for the purpose of distinguishing primary from processed transcri...

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Bibliografische gegevens
Gepubliceerd in:RNA Biol
Hoofdauteurs: Innocenti, Nicolas, Repoila, Francis, Aurell, Erik
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Taylor & Francis 2015
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4615645/
https://ncbi.nlm.nih.gov/pubmed/26177062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2015.1071010
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