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Detection and quantitative estimation of spurious double stranded DNA formation during reverse transcription in bacteria using tagRNA-seq
Standard RNA-seq has a well know tendency to generate “ghost” antisense reads due to formation of spurious second strand cDNA in the sequencing process. We recently reported on a novel variant of RNA-seq coined “tagRNA-seq” introduced for the purpose of distinguishing primary from processed transcri...
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| Publié dans: | RNA Biol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Taylor & Francis
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4615645/ https://ncbi.nlm.nih.gov/pubmed/26177062 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2015.1071010 |
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