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In vitro assay for HCV serine proteinase expressed in insect cells

AIM: To produce the recombinant NS3 protease of hepatitis C virus with enzymatic activity in insect cells. METHODS: The gene of HCV serine proteinase domain which encodes 181 amino acids was inserted into pFastBacHTc and the recombinant plasmid pFBCNS3N was transformed into DH10Bac competent cells f...

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Publicado en:World J Gastroenterol
Autores principales: Hou, Li-Hua, Du, Gui-Xin, Guan, Rong-Bin, Tong, Yi-Gang, Wang, Hai-Tao
Formato: Artigo
Lenguaje:Inglês
Publicado: Baishideng Publishing Group Inc 2003
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4615522/
https://ncbi.nlm.nih.gov/pubmed/12854181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i7.1629
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