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RNA Amplification Protocol Leads to Biased Polymerase Chain Reaction Results Especially for Low-Copy Transcripts of Human Bone Marrow-Derived Stromal Cells

The amplification of RNA is becoming increasingly important, as often only limited amounts of cells are available for gene expression analysis. In this study, the gene expression profile of the 39 human homeobox (HOX) genes was analyzed in bone marrow-derived multipotent stromal cells (BM-MSCs) by r...

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Bibliografski detalji
Izdano u:PLoS One
Glavni autori: Coenen, Carolin, Liedtke, Stefanie, Kogler, Gesine
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4613830/
https://ncbi.nlm.nih.gov/pubmed/26485654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0141070
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