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Creation of Functional Viruses from Non-Functional cDNA Clones Obtained from an RNA Virus Population by the Use of Ancestral Reconstruction

RNA viruses have the highest known mutation rates. Consequently it is likely that a high proportion of individual RNA virus genomes, isolated from an infected host, will contain lethal mutations and be non-functional. This is problematic if the aim is to clone and investigate high-fitness, functiona...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Fahnøe, Ulrik, Pedersen, Anders Gorm, Dräger, Carolin, Orton, Richard J, Blome, Sandra, Höper, Dirk, Beer, Martin, Rasmussen, Thomas Bruun
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2015
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4613144/
https://ncbi.nlm.nih.gov/pubmed/26485566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0140912
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