Expression of Helicobacter pylori Hsp60 protein and its immunogenicity

AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity. METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein. Immunogen...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Bai, Yang, Li, Liang-Ren, Wang, Ji-De, Chen, Ye, Jin, Jian-Feng, Zhang, Zhao-Shan, Zhou, Dian-Yuan, Zhang, Ya-Li
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2003
Assuntos:
H Pylori
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4612038/
https://ncbi.nlm.nih.gov/pubmed/14669319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i12.2711
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