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A Novel ImageJ Macro for Automated Cell Death Quantitation in the Retina

PURPOSE: TUNEL assay is widely used to evaluate cell death. Quantification of TUNEL-positive (TUNEL(+)) cells in tissue sections is usually performed manually, ideally by two masked observers. This process is time consuming, prone to measurement errors, and not entirely reproducible. In this paper,...

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Détails bibliographiques
Publié dans:Invest Ophthalmol Vis Sci
Auteurs principaux: Maidana, Daniel E., Tsoka, Pavlina, Tian, Bo, Dib, Bernard, Matsumoto, Hidetaka, Kataoka, Keiko, Lin, Haijiang, Miller, Joan W., Vavvas, Demetrios G.
Format: Artigo
Langue:Inglês
Publié: The Association for Research in Vision and Ophthalmology 2015
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4611955/
https://ncbi.nlm.nih.gov/pubmed/26469755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.15-17599
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