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Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori
AIM: To construct a recombinant E. coli strain that would highly express the proinflammatory outer membrane protein of human Helicobacter pylori (H pylori). METHODS: The oipA DNA was amplified by PCR, inserted into pET-32a, and transformed into Top10 E. coli strain. This recombinant plasmid of Top10...
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| Publicado no: | World J Gastroenterol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Baishideng Publishing Group Inc
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4611274/ https://ncbi.nlm.nih.gov/pubmed/15457576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i21.3205 |
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