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A novel IgY-Aptamer hybrid system for cost-effective detection of SEB and its evaluation on food and clinical samples

In the present study, we introduce a novel hybrid sandwich-ALISA employing chicken IgY and ssDNA aptamers for the detection of staphylococcal enterotoxin B (SEB). Cloning, expression and purification of the full length recombinant SEB was carried out. Anti-SEB IgY antibodies generated by immunizing...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Mudili, Venkataramana, Makam, Shivakiran S., Sundararaj, Naveen, Siddaiah, Chandranayaka, Gupta, Vijai Kumar, Rao, Putcha V. Lakshmana
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4609960/
https://ncbi.nlm.nih.gov/pubmed/26477645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep15151
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