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Improved two-photon imaging of living neurons in brain tissue through temporal gating

We optimize two-photon imaging of living neurons in brain tissue by temporally gating an incident laser to reduce the photon flux while optimizing the maximum fluorescence signal from the acquired images. Temporal gating produces a bunch of ~10 femtosecond pulses and the fluorescence signal is impro...

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書目詳細資料
發表在:Biomed Opt Express
Main Authors: Gautam, Vini, Drury, Jack, Choy, Julian M. C., Stricker, Christian, Bachor, Hans-A., Daria, Vincent R.
格式: Artigo
語言:Inglês
出版: Optical Society of America 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4605060/
https://ncbi.nlm.nih.gov/pubmed/26504651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.6.004027
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