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Improved two-photon imaging of living neurons in brain tissue through temporal gating
We optimize two-photon imaging of living neurons in brain tissue by temporally gating an incident laser to reduce the photon flux while optimizing the maximum fluorescence signal from the acquired images. Temporal gating produces a bunch of ~10 femtosecond pulses and the fluorescence signal is impro...
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| Pubblicato in: | Biomed Opt Express |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Optical Society of America
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4605060/ https://ncbi.nlm.nih.gov/pubmed/26504651 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.6.004027 |
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