Simultaneous FRAP, FLIM and FAIM for measurements of protein mobility and interaction in living cells

We present a novel integrated multimodal fluorescence microscopy technique for simultaneous fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging (FLIM) and fluorescence anisotropy imaging (FAIM). This approach captures a series of polarization-resolved fluorescence lifeti...

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Bibliographic Details
Published in:Biomed Opt Express
Main Authors: Levitt, James A., Morton, Penny E., Fruhwirth, Gilbert O., Santis, George, Chung, Pei-Hua, Parsons, Maddy, Suhling, Klaus
Format: Artigo
Language:Inglês
Published: Optical Society of America 2015
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4605044/
https://ncbi.nlm.nih.gov/pubmed/26504635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.6.003842
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