Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion.

Amino acid residues D24/D25, E99/E100, E360/E361, and D363/E364 in subdomain 1 of Dictyostelium actin were replaced with histidine residues by site-directed mutagenesis. Mutant actins were expressed in Dictyostelium cells and purified to homogeneity. The sliding movement of mutant actin filaments on...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Johara, M, Toyoshima, Y Y, Ishijima, A, Kojima, H, Yanagida, T, Sutoh, K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46038/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8460118/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.6.2127
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