An improved method for detecting circulating microRNAs with S-Poly(T) Plus real-time PCR

We herein describe a simple, sensitive and specific method for analysis of circulating microRNAs (miRNA), termed S-Poly(T) Plus real-time PCR assay. This new method is based on our previously developed S-Poly(T) method, in which a unique S-Poly(T) primer is used during reverse-transcription to incre...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Niu, Yanqin, Zhang, Limin, Qiu, Huiling, Wu, Yike, Wang, Zhiwei, Zai, Yujia, Liu, Lin, Qu, Junle, Kang, Kang, Gou, Deming
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4602224/
https://ncbi.nlm.nih.gov/pubmed/26459910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep15100
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