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Computational analysis of stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding

The polymerase chain reaction (PCR) is one of the most widely used techniques in molecular biology. In combination with High Throughput Sequencing (HTS), PCR is widely used to quantify transcript abundance for RNA-seq, and in the context of analysis of T and B cell receptor repertoires. In this stud...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Best, Katharine, Oakes, Theres, Heather, James M., Shawe-Taylor, John, Chain, Benny
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4602216/
https://ncbi.nlm.nih.gov/pubmed/26459131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep14629
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