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Reliable and inexpensive expression of large, tagged, exogenous proteins in murine bone marrow-derived macrophages using a second generation lentiviral system

Over the past two decades, researchers have struggled to efficiently express foreign DNA in primary macrophages, impeding research progress. The applications of lipofection, electroporation, microinjection, and viral-mediated transfer typically result in disruptions in macrophage differentiation and...

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Detalhes bibliográficos
Publicado no:J Biol Methods
Main Authors: Miller, Matthew R., Blystone, Scott D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4598941/
https://ncbi.nlm.nih.gov/pubmed/26457319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2015.66
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