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High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria

Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS), it is debated if they are also the major cellular sources of reactive oxygen species (ROS). Here we describe the novel approach of combining high-resolution respirometry and fluorometric measurement...

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Detalhes bibliográficos
Publicado no:Biomolecules
Main Authors: Makrecka-Kuka, Marina, Krumschnabel, Gerhard, Gnaiger, Erich
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4598754/
https://ncbi.nlm.nih.gov/pubmed/26131977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom5031319
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