Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

BACKGROUND: We have devised a simple and efficient fluorescence-based method to track antigen uptake and processing in human B lymphoblastoid cells (B-LCL). Fluorescein labelled subtilisin was used to optimize antigen uptake conditions and identify processed peptides from human cell lines. RESULTS:...

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Detalhes bibliográficos

Publicado no:	BMC Immunol
Principais autores:	Patil, Namrata S, Wong, David L, Collier, Katherine D, McDonald, Hugh C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2004
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC459215/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15222895/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2172-5-12
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