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Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy

Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful quantitative study of this issue is missing. In this paper, we show th...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wu, Yong, Wu, Xundong, Lu, Rong, Zhang, Jin, Toro, Ligia, Stefani, Enrico
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4589784/
https://ncbi.nlm.nih.gov/pubmed/26424175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep14766
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