Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific insertion of a trait gene using clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)-guide RNA technology are reported in maize. DNA vectors expressing maize codon-optimized Streptoco...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Plant Physiol
Hauptverfasser: Svitashev, Sergei, Young, Joshua K., Schwartz, Christine, Gao, Huirong, Falco, S. Carl, Cigan, A. Mark
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society of Plant Biologists 2015
Schlagworte:
Breakthrough Technologies
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4587463/
https://ncbi.nlm.nih.gov/pubmed/26269544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.15.00793
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