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Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording

Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induc...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Amendola, Julien, Boumedine, Norah, Sangiardi, Marion, El Far, Oussama
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4585864/
https://ncbi.nlm.nih.gov/pubmed/26399440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep14455
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