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High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.

We report here a 100-fold increased frequency of plastid transformation in tobacco by selection for a chimeric aadA gene encoding aminoglycoside 3"-adenylyltransferase, as compared with that obtained with mutant 16S rRNA genes. Expression of aadA confers resistance to spectinomycin and streptomycin....

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Svab, Z, Maliga, P
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45780/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8381537/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.3.913
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