A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA

Gene targeting (GT) is a useful technology for accurate genome engineering in plants. A reproducible approach based on a positive-negative selection system using hygromycin resistance and the diphtheria toxin A subunit gene as positive and negative selection markers, respectively, is now available....

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Bibliographic Details
Published in:Plant Physiol
Main Authors: Nishizawa-Yokoi, Ayako, Nonaka, Satoko, Osakabe, Keishi, Saika, Hiroaki, Toki, Seiichi
Format: Artigo
Language:Inglês
Published: American Society of Plant Biologists 2015
Subjects:
Breakthrough Technologies
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4577407/
https://ncbi.nlm.nih.gov/pubmed/26143254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.15.00638
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