Assembly, translocation, and activation of XerCD-dif recombination by FtsK translocase analyzed in real-time by FRET and two-color tethered fluorophore motion

The FtsK dsDNA translocase functions in bacterial chromosome unlinking by activating XerCD-dif recombination in the replication terminus region. To analyze FtsK assembly and translocation, and the subsequent activation of XerCD-dif recombination, we extended the tethered fluorophore motion technique...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: May, Peter F. J., Zawadzki, Pawel, Sherratt, David J., Kapanidis, Achillefs N., Arciszewska, Lidia K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2015
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PNAS Plus
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4577173/
https://ncbi.nlm.nih.gov/pubmed/26324908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1510814112
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