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Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification

We report herein the development of a highly sensitive and selective approach for label-free DNA detection by combining target-recycled ligation (TRL), magnetic nanoparticle assisted target capture/separation, and efficient enzymatic amplification. We show that our approach can detect as little as 3...

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Detalhes bibliográficos
Publicado no:Nanoscale
Main Authors: Zhang, Yue, Guo, Yuan, Quirke, Philip, Zhou, Dejian
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4576341/
https://ncbi.nlm.nih.gov/pubmed/23636707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3nr01010d
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