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Detection of RNA nucleoside modifications with the uridine-specific ribonuclease MC1 from Momordica charantia

A codon-optimized recombinant ribonuclease, MC1 is characterized for its uridine-specific cleavage ability to map nucleoside modifications in RNA. The published MC1 amino acid sequence, as noted in a previous study, was used as a template to construct a synthetic gene with a natural codon bias favor...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:RNA
Hauptverfasser: Addepalli, Balasubrahmanym, Lesner, Nicholas P., Limbach, Patrick A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4574751/
https://ncbi.nlm.nih.gov/pubmed/26221047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.052472.115
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