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RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling

RESOLFT super-resolution microscopy allows subdiffraction resolution imaging of living cells using low intensities of light. It relies on the light-driven switching of reversible switchable fluorescent proteins (RSFPs). So far, RESOLFT imaging was restricted to living cells, because chemical fixatio...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ilgen, Peter, Grotjohann, Tim, Jans, Daniel C., Kilisch, Markus, Hell, Stefan W., Jakobs, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4574475/
https://ncbi.nlm.nih.gov/pubmed/26375606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0136233
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