Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system

BACKGROUND: Direct cloning combined with heterologous expression of a secondary metabolite biosynthetic gene cluster has become a useful strategy for production improvement and pathway modification of potentially valuable natural products present at minute quantities in original isolates of actinomy...

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Bibliografiska uppgifter
I publikationen:Microb Cell Fact
Huvudupphovsmän: Nah, Hee-Ju, Woo, Min-Woo, Choi, Si-Sun, Kim, Eung-Soo
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2015
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4573296/
https://ncbi.nlm.nih.gov/pubmed/26377404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0325-2
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