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Simplified purification method for Clostridium difficile toxin A

AIM: To establish the purification method for Clostridium difficile (C. difficile) toxin A. METHODS: C. difficile VPI 10463 filtrate was cultured anaerobically by the dialysis bag methods. And then the toxin A was purified by precipitation with 500 g/L (NH(4))(2)SO(4) and acid precipitation at pH5.5...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Fu, Si-Wu, Xue, Jing, Zhang, Ya-Li, Zhou, Dian-Yuan
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4572210/
https://ncbi.nlm.nih.gov/pubmed/15309736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i18.2756
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