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In situ structural analysis of Golgi intracisternal protein arrays

We acquired molecular-resolution structures of the Golgi within its native cellular environment. Vitreous Chlamydomonas cells were thinned by cryo-focused ion beam milling and then visualized by cryo-electron tomography. These tomograms revealed structures within the Golgi cisternae that have not be...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Engel, Benjamin D., Schaffer, Miroslava, Albert, Sahradha, Asano, Shoh, Plitzko, Jürgen M., Baumeister, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4568700/
https://ncbi.nlm.nih.gov/pubmed/26311849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1515337112
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