Screening for recombinants of Crambe abyssynica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying dexamethasone (DEX) can activate the recombinase to remove the T-DNA fragment between reco...

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Published in:Sci Rep
Main Authors: Qi, Weicong, Tinnenbroek-Capel, Iris E. M., Salentijn, Elma M. J., Schaart, Jan G., Cheng, Jihua, Denneboom, Christel, Zhang, Zhao, Zhang, Xiaolin, Zhao, Han, Visser, Richard G. F., Huang, Bangquan, Van Loo, Eibertus N., Krens, Frans A.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4566089/
https://ncbi.nlm.nih.gov/pubmed/26358007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep14033
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