Improved rapid and efficient method for Staphylococcus aureus DNA extraction from milk for identification of mastitis pathogens

A rapid and efficient DNA extraction method was developed for detecting mastitis pathogens in milk. The first critical step involved cell wall disruption by bead-beating, as physical disruption using beads was more effective for DNA extraction from Gram-positive bacteria, such as Staphylococcus aure...

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Bibliographic Details
Published in:J Vet Med Sci
Main Authors: UNNO, Hirotaka, INADA, Mika, NAKAMURA, Akiyoshi, HASHIMOTO, Michie, ITO, Keiko, HASHIMOTO, Koji, NIKAIDO, Masaru, HAYASHI, Tomohito, HATA, Eiji, KATSUDA, Ken, KIKU, Yoshio, TAGAWA, Yuichi, KAWAI, Kazuhiro
Format: Artigo
Language:Inglês
Published: The Japanese Society of Veterinary Science 2015
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4565803/
https://ncbi.nlm.nih.gov/pubmed/25843742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1292/jvms.14-0159
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