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Bovine pancreatic trypsin inhibitor-trypsin complex as a detection system for recombinant proteins.

Bovine pancreatic trypsin inhibitor (BPTI) binds to trypsin and anhydrotrypsin (an enzymatically inactive derivative of trypsin) with affinities of 6 x 10(-14) and 1.1 x 10(-13) M, respectively. We have taken advantage of the high affinity and specificity of this binding reaction to develop a protei...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Borjigin, J, Nathans, J
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1993
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45655/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7678346/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.1.337
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