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Improved DNase-seq protocol facilitates high resolution mapping of DNase I hypersensitive sites in roots in Arabidopsis thaliana

BACKGROUND: Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high throughput sequencing to identi...

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Bibliographic Details
Published in:Plant Methods
Main Authors: Cumbie, Jason S., Filichkin, Sergei A., Megraw, Molly
Format: Artigo
Language:Inglês
Published: BioMed Central 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4558764/
https://ncbi.nlm.nih.gov/pubmed/26339280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-015-0087-1
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