PEA-CLARITY: 3D molecular imaging of whole plant organs

Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins...

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Vydáno v:Sci Rep
Hlavní autoři: Palmer, William M., Martin, Antony P., Flynn, Jamie R., Reed, Stephanie L., White, Rosemary G., Furbank, Robert T., Grof, Christopher P. L.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4556961/
https://ncbi.nlm.nih.gov/pubmed/26328508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13492
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