Detection of theileriosis in cattle and buffaloes by polymerase chain reaction

Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theiler...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:J Parasit Dis
Main Authors: Kundave, V. R., Patel, A. K., Patel, P. V., Hasnani, J. J., Joshi, C. G.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer India 2013
Assuntos:
Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4554556/
https://ncbi.nlm.nih.gov/pubmed/26345061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12639-013-0386-2
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-4554556
record_format dspace
spelling pubmed-45545562016-09-01 Detection of theileriosis in cattle and buffaloes by polymerase chain reaction Kundave, V. R. Patel, A. K. Patel, P. V. Hasnani, J. J. Joshi, C. G. J Parasit Dis Original Article Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes. Springer India 2013-11-19 2015-09 /pmc/articles/PMC4554556/ /pubmed/26345061 http://dx.doi.org/10.1007/s12639-013-0386-2 Text en © Indian Society for Parasitology 2013
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Original Article
spellingShingle Original Article
Kundave, V. R.
Patel, A. K.
Patel, P. V.
Hasnani, J. J.
Joshi, C. G.
Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
description Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes.
author Kundave, V. R.
Patel, A. K.
Patel, P. V.
Hasnani, J. J.
Joshi, C. G.
author_facet Kundave, V. R.
Patel, A. K.
Patel, P. V.
Hasnani, J. J.
Joshi, C. G.
author_sort Kundave, V. R.
title Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
title_short Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
title_full Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
title_fullStr Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
title_full_unstemmed Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
title_sort detection of theileriosis in cattle and buffaloes by polymerase chain reaction
publisher Springer India
container_title J Parasit Dis
publishDate 2013
url https://ncbi.nlm.nih.gov/pmc/articles/PMC4554556/
https://ncbi.nlm.nih.gov/pubmed/26345061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12639-013-0386-2
_version_ 1809542416369188864

Registos relacionados