A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas

B cell hybridomas are an important source of monoclonal antibodies. In this paper, we developed a high-throughput method to characterize mouse IgG antibodies using surface plasmon resonance technology. This assay rapidly determines their sub-isotypes, whether they bind native antigen and their appro...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Liu, Haolin, White, Janice, Crawford, Frances, Jin, Niyun, Ju, Xiangwu, Liu, Kangtai, Jiang, Chengyu, Marrack, Philippa, Zhang, Gongyi, Kappler, John W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4552657/
https://ncbi.nlm.nih.gov/pubmed/26317987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0136613
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