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Simplified three-dimensional culture system for long-term expansion of embryonic stem cells

AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells (ESCs) labeled with enhanced yellow fluorescent protein were cultured in three-dimensional (3-D) self-assembling scaff...

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Bibliographic Details
Published in:World J Stem Cells
Main Authors: McKee, Christina, Perez-Cruet, Mick, Chavez, Ferman, Chaudhry, G Rasul
Format: Artigo
Language:Inglês
Published: Baishideng Publishing Group Inc 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4550630/
https://ncbi.nlm.nih.gov/pubmed/26328022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4252/wjsc.v7.i7.1064
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