Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT(™)) and standard density gradient

BACKGROUND: High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extract...

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Udgivet i:BMC Immunol
Main Authors: Corkum, Christopher P., Ings, Danielle P., Burgess, Christopher, Karwowska, Sylwia, Kroll, Werner, Michalak, Tomasz I.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2015
Fag:
Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4549105/
https://ncbi.nlm.nih.gov/pubmed/26307036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12865-015-0113-0
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