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Different Donor Cell Culture Methods Can Influence the Developmental Ability of Cloned Sheep Embryos

It was proposed that arresting nuclear donor cells in G0/G1 phase facilitates the development of embryos that are derived from somatic cell nuclear transfer (SCNT). Full confluency or serum starvation is commonly used to arrest in vitro cultured somatic cells in G0/G1 phase. However, it is controver...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ma, LiBing, Liu, XiYu, Wang, FengMei, He, XiaoYing, Chen, Shan, Li, WenDa
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4546374/
https://ncbi.nlm.nih.gov/pubmed/26291536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0135344
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