DNA supercoiling changes the spacing requirement of two lac operators for DNA loop formation with lac repressor.

We have used a gel retardation assay to investigate the influence of DNA supercoiling on loop formation between lac repressor and two lac operators. A series of 15 DNA minicircles of identical size (452 bp) was constructed carrying two lac operators at distances ranging from 153 to 168 bp. Low posit...

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Enregistré dans:
Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: Krämer, H, Amouyal, M, Nordheim, A, Müller-Hill, B
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 1988
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC454353/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2835234/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02844.x
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