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DNA supercoiling changes the spacing requirement of two lac operators for DNA loop formation with lac repressor.
We have used a gel retardation assay to investigate the influence of DNA supercoiling on loop formation between lac repressor and two lac operators. A series of 15 DNA minicircles of identical size (452 bp) was constructed carrying two lac operators at distances ranging from 153 to 168 bp. Low posit...
Enregistré dans:
| Publié dans: | EMBO J |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
1988
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC454353/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2835234/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02844.x |
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