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DNA supercoiling changes the spacing requirement of two lac operators for DNA loop formation with lac repressor.

We have used a gel retardation assay to investigate the influence of DNA supercoiling on loop formation between lac repressor and two lac operators. A series of 15 DNA minicircles of identical size (452 bp) was constructed carrying two lac operators at distances ranging from 153 to 168 bp. Low posit...

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Bibliografski detalji
Glavni autori: Krämer, H, Amouyal, M, Nordheim, A, Müller-Hill, B
Format: Artigo
Jezik:Inglês
Izdano: 1988
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC454353/
https://ncbi.nlm.nih.gov/pubmed/2835234
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