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Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Indoxyl glycosides proved to be valuable and versatile tools for monitoring glycosidase activities. Indoxyls are released by enzymatic hydrolysis and are rapidly oxidized, for example by atmospheric oxygen, to indigo type dyes. This reaction enables fast and easy screening in vivo without isolation...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Böttcher, Stephan, Thiem, Joachim
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542957/
https://ncbi.nlm.nih.gov/pubmed/26068577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52442
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