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Primary Tumor and MEF Cell Isolation to Study Lung Metastasis

In breast tumorigenesis, the metastatic stage of the disease poses the greatest threat to the affected individual. Normal breast cells with altered genotypes now possess the ability to invade and survive in other tissues. In this protocol, mouse mammary tumors are removed and primary cells are prepa...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Dong, Shengli, Maziveyi, Mazvita, Alahari, Suresh K.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542731/
https://ncbi.nlm.nih.gov/pubmed/26066699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52609
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